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or fifteen minutes, the remainder of the liquid is poured into an appropriately small cup and heated to 65" Celsius. New aquaeous vapor will be forming and the temperature must be kept at 65° C., as long as this is the case. As soon as the formation of vapor ceases, we may assume that very little water lias remained. To determine, however, accurately whether or not the rest of the liquid contains water, the following delicate test will be indispensable: By placing an ordinary thermometer with a long and narrow mercury bulb into the liquid and rapidly withdrawing it just at 65IJ ('., we will probably see a puff of vapor ascending from the mercury bulb, which is an indication that some water is still present in the remainder of the urine. This procedure is repeated at short intervals and finally we will arrive to a point, when there is no more vapor ascending from the mercury-bulb. Now we can feel certain that most, or rather nearly all of the remaining liquid is nothing less than the heretofore unknown component of the urine. We now measure in a small measure glass the amount of organic liquid obtained and add to it the same amount of absolute alcohol together with 0.5 (five decigramm) of pure, powdered oxalic acid for each 100 Cubic centimeter of urine evaporated. The newly formed Urea-Oxalate being practically insoluble in such a small quantity of alcohol, can be easily separated together with most of the solid ingredients of the urine by filtrating the alcoholic liquid through a ten small filter, provided, however, that the same (the liquid) has been allowed to cool off sufficiently. Should a new precipitate form in the filtrate, an additional small quantity of alcohol should be added to it and the whole should be filtered again through the old filter in order not to lose unnecessarily any however small portion of the new organic substance; for the same reason it is advisable to wash out carefully the first and second recipient with a little alcohol, which is to be added to the referred to.
We now evaporate carefully the alcoholic solution of our organic body, until every trace of alcohol has disappeared. When the presence of alcohol is no more noticeable either through smell or through a slight vapor, we let stand the remaining reddish-brown liquid until cooled off and turn around and around the latter for some length of time in its recipient, until all solid particles of urea and salts have separated and have stuck to the sides of the cup. There is finally nothing left in the recipient but the organic body in question together with the coloring matters of the urine, principally the Urochroin . To separate the coloring matters from our organic body, the latter must be treated with a saturated solution of Mercury Nitrate (Mercuric, Ug(N< until no further precipitate is forming. Filter again and evaporate carefully, resorting at short intervals to the test above referred to (heating to 65" C. and examining with longmercury-bulb thermometer as to the formation of vapor). The liquid thus obtained resembles in aspect olive-oil, is of a pale yellow color, of a sligthU bitter taste, gives to the touch the impression of a fart y substance, produces on paper spots resembling fat spots, though not so markedly as spots produced by fat. Its specific gravity is about 1,065. Its quantity is about double that of urea.
I have found as much as 75 (seventy-five) gramms in 24 hours, and as
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