1837 government stipend for five years' study at the Königlich Medizinisch-chirurgische Friedrich-Wilhelm-Institut in Berlin; in return Helmholtz comitted himself to eight years' service as an army surgeon; 1838 entry into the University of Berlin, studies of chemistry under Eilhardt Mitscherlich, of clinical medicine under Lucas Schönlein, and of physiology under Johannes Müller; 1841 research for his dissertation under Johannes Müller and later move into the circle of Müller's students (chief among these were Ernst Brücke and Emil Du Bois-Reymond); with Carl Ludwig they made up the '1847 school' of physiology; M.D. degree in 1842; after completing the state medical examinations appointment as surgeon to the regiment at Potsdam; in 1845 DuBois-Reymond brought him into the newly founded Physikalische Gesellschaft; release from his military duty; 1849 associate professor of physiology at Königsberg; 1855 transfer to the chair of anatomy and physiology at Bonn; 1858 chair at Heidelberg; 1871 chair of physics at Berlin; 1887 assumption of the presidency of the newly founded Imperial Physico-Technical Institution for research in the exact sciences and precision technology.
De Fabrica Systematis Nervosi Evertebratorum (Diss.), Berlin 1842; Über die Erhaltung der Kraft, Berlin 1847; Messungen über den zeitlichen Verlauf der Zuckung animalischer Muskeln und die Fortpflanzungsgeschwindigkeit der Reizung in den Nerven, in: A. Anat. Physiol. 277(1850); Über die Wechselwirkung der Naturkräfte, Königsberg 1854; Handbuch der physiologischen Optik, 3 Bde., Leipzig 1855-1867; Die Lehre von den Tonempfindungen als physiologische Grundlage für die Theorie der Musik, Brunswick 1863 (≤1865, 3. umgearb. Ausg. 1870).
Handbuch der physiologischen Optik (1856-66, reissued together in 1867; English translation, Treatise on hysiologiscal Optics, 1925)